Golgi method for old formol-fixed human foetal tissue.

Differentiation of foetal and maternal erythrocytes in formol-fixed tissues.

Human intestinal mucosal mast cells: evaluation of fixation and staining techniques.

The staining properties of tissue mast cells are influenced by the method of fixation. Differences in fixation and staining techniques may explain the contradictory results in the published reports on the number of human mucosal mast cells (MMC) in the gastrointestinal mucosa in health and disease. We have examined the influence of fixatives on the staining properties of human MMC in operative ...

Electron Microscope Observations on Frozen-dried Cells

To explore the problem of artefacts which may be produced during usual fixation, dehydration, and embedding, the authors have examined pancreas, liver, and bone marrow frozen at about -180 degrees C., dried, at -55 to -60 degrees C., embedded in methacrylate, sectioned, and floated on a formol-alcohol mixture. By these treatments the labile structure of living cells can be fixed promptly and em...

Polarization Optical Properties of the Pancreatic Acinar Cell of the Mouse

The birefringence of fresh and fixed mouse pancreatic acinar tissue was studied, utilizing whole mounts of pancreas from which the mesentery had been removed. Fresh pancreas in Tyrode's solution demonstrated positive birefringence with respect to the radial axis (the axis radiating from the nucleus as spokes from a wheel). Formol fixation reversed the sign of birefringence to negative with resp...

Effects of fixation and processing on immunohistochemical demonstration of immunoglobulin in paraffin sections of tonsil and bone marrow.

A number of fixatives were tested to determine their suitability for use with the unlabelled antibody peroxidase-antiperoxidase (PAP) method for demonstrating immunoglobulin in paraffin sections of tonsil and trephine samples of bone marrow. It was found that tonsil fixed in 'isotonic' solutions of formaldehyde reacted with the PAP method only after the sections had been trypsinised. Several ot...